In vitro Regeneration Studies of Vuralia turcica Using Unpollinated Ovaries

Vuralia turcica is an endemic plant species with a polycarpellary gynoecium and has the ability to survive in salt stress. However, maintenance of this currently challenging because climatic changes plant’s habitat, intensive agricultural purposes, grazing, like. Gynogenesis promising method for micropropagation useful technic genetic engineering. The objective study was analyze gynogenesis V. turcica. experiments described here were implemented during flowering time (spring) using unpollinated ovaries. Modified MS B5 induction media different growth regulators (BAP, GA3, 2,4-D, KIN) compared. comparison both (MS B5) showed that medium supplemented 2,4-D KIN best vitro unfertilized ovary culture gave result regenerated plants per 30 ovaries, 92% callus obtained. Only control groups very low scores or no plants. From regeneration subcultures, total 60 produced, all green. These results are interest conservation endangered like